Adenosine 3':5'-monophosphate content and actions in the division cycle of synchronized HeLa cells.

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Adenosine 3':5'-monophosphate content and actions in the division cycle of synchronized HeLa cells

The involvement of adenosine 3':5'-monophosphate (cAMP) in the regulation of the cell cycle was studied by determining intracellular fluctuations in cAMP levels in synchronized HeLa cells and by testing the effects of experimentally altered levels on cell cycle traverse. Cyclic AMP levels were lowest during mitosis and were highest during late G-1 or early S phase. These findings were supported...

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Progression of the HeLa cell through its life cycle is accompanied by centriolar replication and pericentriolar changes that are in synchrony with DNA synthesis and mitosis. The first signs of preparation for replication occur during G(1) at which time the two orthogonal centrioles separate. Replication by budding begins at/or near the initiation of DNA synthesis and is completed by G(2). Peric...

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Measurements of actinomycin-3H binding in synchronized HeLa cells reveal that the binding capacity of chromatin decreases progressively during the S phase despite a doubling of nuclear DNA content, reaches a minimal level during G 3 and mitosis, and then increases gradually throughout the subsequent G, interval . Since this pattern was evident in experiments with living cells, ethanol-fixed cel...

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During the Division Cycle of Hela Cells

The lethal actions of mitomycin C and actinomycin D were followed during the division cycle of HeLa cells. The cells were most susceptible to a 2 hr pulse of mitomycin C during the G1 phase, whereas their sensitivity to actinomycin D was most pronounced in the S phase. Posttreatment of the cells with acetoxycycloheximide, a potent inhibitor of protein synthesis, increased the survival (colony-f...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1976

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.71.2.515